pntd.0009322.s008.tif (1.28 MB)
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posted on 2021-04-08, 18:02 authored by Ana K. Ibarrola-Vannucci, Luis M. De Pablos, Lissette Retana-Moreira, Alberto Cornet-Gomez, Teresa Cruz-Bustos, Alejandro G. Schijman, José L. Ramírez, Susana Vílchez, Antonio OsunaA. Circular map of the pTREX-TAPtag-GW vector: The GATEWAY (GW) cassette is shown in green and the TAPtag is shown in light blue, which consists of CBP (calmodulin-binding peptide), PA (protein A) and the cleavage site for TEV protease. The ribosomal promoter is seen in lilac and the HX1 region downstream in red. This plasmid possesses the resistance genes amp (ampicillin) and neo (neomycin). Flanking the neo gene are intergenic gapdh regions, according with [62,53]. B. Localization of PHB1 overexpressed in the epimastigote form and recognized by Protein A labeled with Au 20 nm. C. Localization of PHB2 overexpressed in the epimastigote form and recognized by Protein A labeled with Au 20 nm.
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bioinformatics analysisTrypanosoma cruzi Background Chagas...metacyclic trypomastigotescruzi clonesPHB 2PHB 2 parasitesside effectsnon-infective formsPHB 1 parasitesproteins Prohibitin 1ROSelectron microscopytransformation rateimmuno-electron microscopylife cyclePHB genespleiotropic functiontransfected Tintracellular multiplicationmetacyclic infective formsintracellular amastigotesCRISPRnon-infective replicative epimastig...cruzi PHBs
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