Rottlerin decreases the number of effector cells that mainly infiltrate the skin in IMQ-treated mice.

Immunohistochemical detection of immune cell-related markers was performed on paraffin-embedded sections obtained from the back skin of IMQ-induced mice treated with vehicle or rottlerin. (A–C) Representatives IHC images of CD3 (A), CD11b (B), and CD11c (C) on the skin of the vehicle or rottlerin-treated mice. Scale bar = 100μm. (D–F) Quantification analysis of IHC staining for CD3(D), CD11b(E), and CD11c (F) on the skin of the vehicle and rottlerin treated mice. Two independent researchers counted the number of positive staining cells were per high-power field (HPF). The data are representative of three experiments (n = 5 mice per group).*P<0.05, ** P<0.01 vs. vehicle.