Related to Fig 4.

A) Left: representative images of nuclei (DAPI, blue in merged) subject to immunofluorescence for ERα (green in merge) in MCF-7 cells treated with vehicle, E2 or 4OH for 30 min and pre-extracted with 0.5% Triton-X-100 before fixing. Scale bar, 10 μM. Right: boxplots showing the integrated nuclear intensity. Two-sided Mann-Whitney test, Holm-Bonferroni correction for multiple testing. B and C) Biological replicate of data in Fig 4B and 4C. D) Violin plots showing the distribution of DNA FISH enhancer-promoter interprobe distances at the GREB1 or NRIP1 loci in cells treated as indicated. Boxes indicating the median distances. Two-sided Mann-Whitney test, Holm-Bonferroni correction for multiple testing. Biological replicate for the data in Fig 4E. n.s p> 0.05, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. E) Violin plots showing the distribution of DNA FISH enhancer-control interprobe at GREB1 or NRIP1 loci for cells treated with vehicle, FLV or TRP for 5 min prior to treatment without (-E2) and with E2 (+E2) for 30 min. Boxes indicating the median distances. Two-sided Mann-Whitney test, Holm-Bonferroni correction for multiple testing shows no significant differences. Data from two biological replicates shown. Statistical data for are in S4 Table.

(EPS)