Reductions in vaccinia virus replication do not correlate with markers of cell viability.

(A) Brightfield microscope images at 10X of A549 cells grown in glucose/galactose with and without interferon priming; uninfected (top) and vaccinia virus infected (bottom). (B) Brightfield microscope images at 20X of A549 cells grown in glucose/galactose with and without interferon priming; uninfected (top) and vaccinia virus infected (bottom). (C) PARP cleavage western blots of uninfected A549 cells grown in glucose/galactose with and without interferon priming. (D) PARP cleavage western blots of vaccinia virus infected A549 cells grown in glucose/galactose with and without interferon priming. Control: VSV-infected glucose/galactose cells (no interferon priming). Glc: glucose (25 mM). Gal: galactose (10 mM). Loading control: β-actin. A27: vaccinia virus late protein. IFN-α or IFN-γ concentration: 1000 units/mL.