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Reduction of the PDI-M catalytic domains by AtNTRB as assayed using DTNB and insulin.
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posted on 2017-03-31, 18:10 authored by Benjamin Selles, Flavien Zannini, Jérémy Couturier, Jean-Pierre Jacquot, Nicolas RouhierA. DTNB reduction assay. Representative kinetic curves obtained in the presence of NADPH and AtNTRB alone or with 0.2 μM PDI-M, PDI-M(a°) or PDI-M(a). B. Insulin reduction assessed using NADPH/AtNTRB as an electron donor system and 5 μM of PDI-M, PDI-M(a°) or PDI-M(a).
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Fe 2 S 2reduction reactions-170 mVmoduleAtypical protein disulfide isomerasesNADPH thioredoxin reductasepH 7.0.oxidationWCKHCPDI-L 1A Protein disulfide isomerasesnon-physiological substrate NADP-MDHb domains influencemulti-modular redox catalystspoplar PDI isoformsdithiol-disulfide exchange reactionsPDI-L 1aEscherichia coli expressionPDI-APDI-MNADP malate dehydrogenase
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