Recombinant M-CDK specifically phosphorylates SP motifs in H1.1.

Wild-type (WT) and the 5SA mutant of H1.1 were incubated with M-CDK and ATP at 25°C. Aliquots were taken at the indicated time points and subjected to conventional SDS-PAGE. The gel was first stained with Pro-Q Diamond solution to visualize phosphorylated proteins and then stained with CBB to visualize total proteins (a pair of upper panels). The same set of samples was also subjected to Phos-tag SDS-PAGE (a lower panel).

(EPS)