Ras-induced EpCAM and several other membrane proteins.

A. Microarray data (GSE12777) for 51 breast cancer cell line was analyzed using Gene-E software. E-cadherin correlated 121 genes are enriched in first step followed by EpCAM close (correlated). These genes are presented in normal and Ras-transduced cells (right panel). B-C. Two independent microarray data sets from MCF10A and Ras-transduced cells were analyzed for EpCAM expression. D. Normalized Microarray data (GSE3151, GSE12764) shows compared to oncogenes myc, Src, B-catenin, E2F3 and MEK, Ras- transduced cells show EpCAM overexpression. E. Immunoblot assay after 96h of transiently transfected MCF10A cells with GFP, Myc, Src, B-Catenin, E2F3 MEK1 and H-Ras shows, H-Ras induced expression of EpCAM.