Rare chiasmata observed in zhp-4(vv96) mutants are rmh-1 but not mus-81 dependent.

(A) Representative oocytes from the indicated genotypes showing diakinesis nuclei stained with DAPI (blue) and HTP-3 (red). (B) Quantification of the DAPI bodies in diakinesis nuclei (-1 and -2 oocytes) from germlines of the indicated genotypes. mus-81 mutants invariably exhibit 6 DAPI structures, whereas the distributions in zhp-4;mus-81 double mutants is not different from zhp-4 single mutants mutation (p>0.05). (C) Quantification of the DAPI bodies in diakinesis nuclei (-1 and -2 oocytes) from germlines of the indicated genotypes. rmh-1 mutants show a distribution not different from zhp-4 mutants while the double mutants have an average of about 12 univalents, significantly different from either single mutant distribution (statistical test Kruskal-Wallis followed by Dunn’s multiple comparison test, ns = not significant p>0.05, *** p<0.001. Nuclei scored: zhp-4(vv96) n = 34, mus-81(tm1937) n = 22, zhp-4(vv96);mus-81(tm1937) n = 16, rmh-1(jf92) n = 20, zhp-4(vv96);rmh-1(jf92) n = 16. Scale bars 5 μm.