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RT-PCR analysis results are affected by sampling system, RNA extraction method and the choice of reference gene.
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posted on 2016-08-30, 17:33 authored by Anke Meyer, Federico Paroni, Kathrin Günther, Gitanjali Dharmadhikari, Wolfgang Ahrens, Sørge Kelm, Kathrin MaedlerSIGLEC-7 gene expression profiles of Series B with additional DNAse digestion. Blood was collected from 12 donors; triplicates from each donor were reverse transcribed and experimental duplicates from each sample run in Real-Time PCR reactions. Data were normalized to (A) PPIA, (B) β-ACTIN, (C) β-TUBULIN and (D) to a combination of these 3 reference genes. Results are shown as relative to the randomly chosen subject #1. A-C show means of experimental duplicates ± SE, D shows means of pooled triplicates from each donor.
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gene expression resultsNorgen RNA extraction methodsPre-analytix PAXgene TM RNA extraction systemTUBULINPAX RNA extraction KitsPAXgene TM blood collection systemTempus TM collection tubesRNA concentrationACTINPAX TM collection systemPAXgene TM collection systemblood samplesHuman Blood mRNA IsolationTempus TM collectionTempus TM collection systemsRNA isolation methodsRNA isolation systemPPIASIGLEC -7. ResultsReal-Time PCR analysesgene expression analysesPAXgene TM RNA extraction systemPAXgene TM blood collection method
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