Purification and verification of sso7d.

(A) SDS-PAGE analysis at various stages of the protein purification process. CR means crude lysate after sonication; CL means clarified lysate prior to loading to the Ni-TED column; W means the flowthrough fraction after the wash step; F means the final eluted protein sample; FB means the final protein sample after buffer exchange. (B) Electrophoretic mobility shift assay to detect sso7d binding of oligo duplex.

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