Productivity of the two-stage stirred tank bioreactor (TSB) system (1290 mL wv; 0.29 mL/min) compared with a hypothetical batch process (645 mL each vessel, 1290 mL wv).

A) TY of the continuous cultivation based on TCID₅₀ values by sampling the VB (squares) and the harvest vessel (circles), versus TY of the batch process (dashed lines; upper and lower lines estimated, assuming a maximum TCID₅₀ at time of harvest of 1×10⁸ and 1×10⁷ virions/mL, respectively). B) STY of the continuous cultivation compared to the batch process (same symbols as in Fig A). It is further assumed that the cell growth phase of the batch and the continuous cultivation were identical, and that both vessels of the batch process were harvested at day 3 and day 12 post infection. TY, time yield; STY, space-time yield; VB, virus bioreactor.