Process flow map of the somatic cell nuclear transfer (SCNT) cloning protocol.

The protocol was divided into three stages: the Cell Culture, Egg Preparation, and Cloning stages. Oval shapes represent the start and end steps in the protocol; rectangles indicate steps in the protocol; rectangles with one curved side indicate a delay or wait time; large diamonds indicate quality assurance (QA) steps; and small triangles indicate steps that are constraints to the system. Steps with a colored background must be performed by skilled operators who have received specialized SCNT training. Single-line arrows indicate the flow of material, whereas double lines indicate that material was removed from the system. Steps in this map can be customized for any process.