pone.0225658.g005.tif (341.08 kB)
Principal component analysis for 96.96 Dynamic Array excluding PBLs dataset.
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posted on 2019-12-03, 18:44 authored by Dominika Borowska, Richard Kuo, Richard A. Bailey, Kellie A. Watson, Pete Kaiser, Lonneke Vervelde, Mark P. StevensAnalysis of immune-related genes in bursa, spleen, ileum and caecal tonsils of birds reared on the sibling test (filled markers) and pedigree (no fill markers) indicates heterogeneity within the tissues and no separation based on the farm type.
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multiplexed qPCR 96.96 Fluidigm Dynamic Array6 nanolitre reactionsbroiler chickensPCR-based platformmicrofluidic system96 transcriptstranscript levelstranscriptome dataamplicon sequencingchicken macrophagesRNA-seq analysispreamplification stepsConventional qPCR analysisGut-associated lymphoid tissuesblood cellstranscriptional response89 genesimmune-related genesbiosecurity levelsmultiplexed qPCR-based platformscreen vaccine responseschicken immune-related genesmultiplex qPCR array96 samplesanalysehigh-throughput assaysblood leukocytescurve analysispathogen-specific probesdendritic cells
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