Principal component analysis (PCA) of the metabolomes of flag leaves.

Green, blue, yellow and red colors represent samples at 0, 7, 14 and 28 DAF, respectively. Ellipse, star, triangle and square denote leaf metabolomes of 9311, Qingfengai, Nipponbare and Nongken 58, respectively. PC 1 explains 24.0% of variance distinguishing flag leaves of two japonica from those of two indica. PC 2 accounts for 14.3% of total variance separating leaf samples from different time points.