PpL decreases the DNA content and causes mitochondrial depolarization in Daudi cells being caspase-9 involved.

A-B) Daudi cells incubated with different doses of PpL, OVA or PBS during 72 hs were labeled with PI. A) Representative overlaid histogram of the DNA content using 90 μg of PpL. B) Percentage of cells with decreased DNA content (mean ± SEM). Experiments were performed three times with similar results, (** p<0.01 PpL (60 μg/ml) vs PBS, *** p<0.001 PpL (90 μg/ml) vs PBS.). C) Representative histograms of the DNA content and the percentage of DNA low cells (mean ± SEM). Daudi cells pretreated or not with inhibitors of caspase-8, -9 or -3, were incubated with PpL (100 μg/ml), OVA (100 μg/ml), or PBS for 72 hs and labeled with PI. Experiments were performed three times with similar results, (*** p<0.001 PpL vs PBS and PpL+ caspase-8 inhibitor vs PBS).