Phylogenetic tree of NADP-ME.

(A). Protein sequences were aligned using Mega 5.10 and the phylogenetic tree was constructed by the neighbor joining method. The numbers indicate the statistical significance of each branch obtained by bootstrap analysis. Relative level of the transcripts of NADP-ME genes in maturing soybean embryos (B, D and F) and castor bean endosperms (C, E and G) determined by qRT-PCR. The functionality of primers for GmNADP-ME1.2 and 1.3 was verified using soybean genomic DNA. The primers for GmNADP-ME2.2 also anneals with the splicing version GmNADP-ME2.2spl. The actin genes Glyma04g39380 and Rco30206.m000761 were used as reference in soybean and castor, respectively. The values are the average of at least two independent experiments ± SD. For each transcript, values with the same letter are not significantly different (p < 0.05).