Phylogenetic analysis based on the complete ORF sequences of L, M and S gene segments of Hubei SFTSVs.

(A) Segment shift events were specially marked including S1 (HBHG2020-02), S2 (HBHG2020-08), S3 (HBHG2021-02), S4 (HBHG2022-12), S5 (HBSZ2022-11), S6 (HBHG2022-36), S7 (HBSZ2022-54) and S8 (HBHG2021-4). The bars indicate pure groups of genotypes colored with green (C1), cyan (C2), blue (C3), fuchsia (C4), gray (C5), brown (C6), pink (J1), purple (J2), red (J3), yellow (J4). The ML trees were constructed and tested by bootstrap analysis with 1000 replications, and these phylogenic trees were mid-point rooted. The scale bars (0.005) indicate the number of nucleotide substitutions per site. “*” representing that the phylogenetic branches were supported with greater than 70% bootstrap values. (B) Genotypes of Hubei SFTSVs detected in human sera and the graphic representation of reassortment events in SFTSV genome sequences obtained from patients. The SFTSVs were assigned to different genotypes based on the genetic origin of each segment as determined by phylogenic analysis. Gene segments from top to bottom are L, M, and S.