Phosphoenzyme formation by the pure Spf1p preparation.

Spf1p (1.5 μg) was phosphorylated under identical conditions as those described in Fig 4. Panel A- Acidic gel electrophoresis. Panel B- Coomassie Blue-stained gel. Panel C- Quantitation of EP. Duplicates for each condition are shown. The EP level detected in the EGTA reaction medium was taken as 100%. Error bars show the standard deviation.