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Phosphoenzyme formation by the pure Spf1p preparation.

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posted on 2020-04-30, 17:29 authored by Gerardo R. Corradi, Luciana R. Mazzitelli, Guido D. Petrovich, Paula Grenon, Danny M. Sørensen, Michael Palmgren, Felicitas de Tezanos Pinto, Hugo P. Adamo

Spf1p (1.5 μg) was phosphorylated under identical conditions as those described in Fig 4. Panel A- Acidic gel electrophoresis. Panel B- Coomassie Blue-stained gel. Panel C- Quantitation of EP. Duplicates for each condition are shown. The EP level detected in the EGTA reaction medium was taken as 100%. Error bars show the standard deviation.

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