Pentamidine induces progressive loss of kinetoplast DNA.
Effects of pentamidine treatment for 12, 24 and 48 h on (A) percentage of kinetoplast negative cells, (B) percentage of TUNEL-positive cells and (C) percentage of extended Mitotracker signal positive cells. The inset in A shows an example of a cell with no detectable kinetoplast DNA; error bars, SD; N = 2; scale bar, 5μm. (D) ImageJ analysis. Raw images (left hand panels) and ImageJ outputs (right hand panels) demonstrating that kinetoplasts fall below the threshold of detection (open green circle). Ratios of kinetoplasts to nuclei, total area and total signal (area multiplied by mean intensity) are plotted for cells with detectable kinetoplasts. Red lines, averages; ***, P < 0.001 from Students t-test.