Optimization and analysis of the FANCA PPIN in 293FT cells.

A. Overview of experimental design to determine the FANCA PPIN in 293FT and EndoC-βH3 cells. All experiments were performed as biological duplicates. B. 137 FANCA protein-protein interactions identified from 293FT lysates. Blue node = FANCA bait, Grey nodes = prey proteins interacting with FANCA with a SAINT-determined BFDR ≤ 0.05 and SAINT score ≥ 0.8. C. ClueGO analysis of enriched Reactome Pathways using the 137 high confidence FANCA interactions identified in 293FT. D. Known protein interactions from database imputed on the set of 137 proteins interacting with FANCA with BisoGenet (v3.0.0) using DIP, BIOGRID, HPRD, INTACT, MINT, and BIND protein-protein interaction database sources. Nodes not connected to the largest network are excluded from this view, but the extended view with all nodes is available in S1 Fig. ClusterOne analysis of this network identifies two significantly interconnected clusters (red circles) representing protein complexes corresponding to known biological roles of FANCA in replication and DNA damage response.