OD254 profile of polysome fractionation.
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posted on 2021-11-23, 18:29 authored by Balakumar Srinivasan, Sarbani Samaddar, Sivaram V. S. Mylavarapu, James P. Clement, Sourav Banerjee(A-G) A254 profile obtained from spectrophotometer attached to gradient fractionator shown in Figs 3 and 4. Traces were drawn from original A254 profile obtained from hippocampal cytoplasmic extract treated with MgCl2 (A), RNase (B), EDTA (C), and MgCl2 (D) shown in Fig 3, MgCl2-treated extract from mouse expressing HA-Rpl22 in excitatory neurons from hippocampus (E) shown in Fig 4C and RNase (F) or without RNase (G)–treated extract from mouse expressing HA-Rpl22 in excitatory neurons from hippocampus shown in Fig 4J. (H, I) A254 profile of sucrose density fractions obtained from vehicle (H) or bicuculline (I)–treated cortical neurons. Traces were drawn from these original A254 profiles as shown in Fig 5A and 5B.
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translating rnas facilitatepostsynaptic ampa receptorsp70 s6 kinaseactively translating transcripts3 ′ utrinduced silencing complexenhancing arc translationdiv >< pinduce homeostatic scalinginvoke mirisc remodelingmov10 degradation alonedependent degradation axishomeostatic scalingtripartite complexmov10 axisinvoke downscalingsynaptic scalingdependent translationdependent mannersubsequent degradationwhereas abrogationvia two processestranslational apparatustranslation regulatorsternary interactionsynaptic downscalingsubsequent removalprotein synthesisoverexpressed mov10individual contributioneither translationeither preventse3 ligasecombinatorial action
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