Nuclear localization of GFP-Ku80a and GFP-Ku80c fusions with or without Triton pre-extraction.

(A) Early autogamous cells expressing GFP-Ku80c or GFP-Ku80a fusions imaged after paraformaldehyde fixation with or without a Triton pre-extraction step. Scale bar is 5 μm. Quantification of total cellular protein levels is shown in S10 Fig. (B) Boxplots of GFP fluorescence intensity in developing MACs from the cells shown in A, fixed without or with pre-extraction. For each fixation condition, the values were normalized relative to the mean signal obtained for the GFP-KU80c transformant. For each condition, 28 to 56 developing MACs were analyzed.