NRC3 complement Cf-2/Rcr3/Avr2-, Cf-5/Avr5-, and Cf-9/Avr9-triggered hypersensitive cell death in the nrc2/3/4 knock-out lines.
A) Cf-2/Rcr3/Avr2-, Cf-5/Avr5-, and Cf-9/Avr9-triggered hypersensitive cell death is complemented in the nrc2/3/4 lines with NRC3, while Cf-9/Avr9-triggered hypersensitive cell death is partially complemented with NRC2. Representative N. benthamiana leaves infiltrated with appropriate constructs were photographed 7–10 days after agroinfiltration. The receptor/effector pair tested, Cf-2/Rcr3/Avr2, Cf-5/Avr5, and Cf-9/Avr9, are labelled above the leaf of nrc2/3/4-184.108.40.206. The NRC used for complementation or EV control are labelled on the leaf image, R3a/Avr3a was used as a positive control. B) Quantification of hypersensitive cell death. Cell death was scored based on a 0–7 scale between 7–10 days post infiltration. The results are presented as a dot plot, where the size of each dot is proportional to the count of the number of samples with the same score within each biological replicate. The experiment was independently repeated three times. The columns correspond to the different biological replicates. Significant differences between the conditions are indicated with an asterisk (*). C) Statistical analysis was conducted using besthr R package . The dots represent the ranked data and their corresponding means (dashed lines), with the size of each dot proportional to the number of observations for each specific value (count key below each panel). The panels on the right show the distribution of 100 bootstrap sample rank means, where the blue areas under the curve illustrate the 0.025 and 0.975 percentiles of the distribution. A difference is considered significant if the ranked mean for a given condition falls within or beyond the blue percentile of the mean distribution of the wild-type control.