Multiplex recombination is increased in an Exo VII deletion strain.

(A) Multiplex insertion. (B) SPI. Three different Gentamicin, Zeocin and Blasticidin lagging strand protected cassettes were inserted at three different sites of the P2rx1 gene: 1 cassette, Gentamicin; 2 cassettes, Gentamicin and Zeocin; 3 cassettes, Gentamicin, Zeocin and Blasticidin. The p15A dhfrII lagging strand protected subcloning plasmid was used in the SPI assays. Data points represent averages; error bars indicate standard error of mean (n = 3 for A and B).