Morphologic analysis of pancreata and islets in p8^-/- and p8^+/+ mice.

A1-A4, representative HE stained bright field image of stacked whole pancreata from wild type (A1) and p8-knockout animals (A2). Glucagon-DAB-staining indicated more islets in p8^-/- (A4) compared to p8^+/+ animals (A3). B1-B6, morphologic analysis of beta islets and pancreas from p8^-/- mice and wild type littermates. p8-knockout animals showed statistically significant higher number of islets (B3), islet area (B4, B5), and beta cell mass (B6). No significant differences were found in mean islet size (not shown), pancreas area (B1) and pancreas weight (B2). Results are expressed as mean ± S.D. of islets and pancreata from 12 animals (6 male, 6 female) per group. Scale bars: 1000μm (A1-A2), 100μm (A3-A4); * P<0.05.