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Molecular identification and genetic complementation of the HPS5 gene.

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posted on 18.07.2016 by Li Song, Haopeng Yu, Jinsong Dong, Ximing Che, Yuling Jiao, Dong Liu

(A), Diagrams showing the positions of the mutations in the ERS1 gene and ERS1 protein. Top: the structure of the ERS1 gene. Black box: exons; grey box: 5’ and 3’ UTRs; thin line: introns; thick line: flanking sequences surrounding the ERS1 gene. Bottom: the structure of the ERS1 protein showing the signature domains. (B), Morphology of 7-day-old seedlings and root tips of the WT, hps5, and complementation lines grown on P+ and P- media. (C), Quantitative analyses of root hair density and root hair length of various lines. Values represent means ± SD of three replicates. Asterisks indicate a significant difference from the WT (t-test, P < 0.05). For each measurement, 15–20 seedlings were used.