Mfap1 does not mediate VCP functions in c4da neurons.

(A)—(E) Mfap1 is not required for c4da neuron dendrite pruning defects upon VCP inhibition. C4da neurons expressing CD8GFP, VCP QQ, and either mcherry RNAi as control or mfap1 RNAi under ppk-GAL4 were visualized at 18 h APF, and dendrite pruning defects were quantified.(A) C4da neuron coexpressing VCP QQ and mcherry RNAi at 18 h APF. (B) C4da neuron coexpressing VCP QQ and mfap1 RNAi at 18 h APF. (C) C4da neuron expressing VCP QQ in a mfap1^DsRed/+ background at 18 h APF. (D) Penetrance of dendrite pruning defects in experiments (A—C). Significance was assessed by Fisher’s exact test. (E) Number of primary and secondary dendrites still attached to the cell body in experiments (A—C). Significance was assessed by Wilcoxon’s test. (F), (G) Mfap1 does not mediate TDP-43 relocalization upon VCP inhibition. Third instar larval c4da neurons expressing mCD8::GFP, VCP QQ, and either mcherry RNAi as control or mfap1 RNAi under ppk-GAL4 were stained for TDP-43, and for GFP to visualize neuronal morphology. (F) Larval c4da neuron coexpressing VCP QQ and mcherry RNAi. (G) Larval c4da neuron coexpressing VCP QQ and mfap1 RNAi. Scale bars are 50 μm in (A) and 10 μm in (F).