Mean actual observed zygosity of OCI-AML3 cells compared to zygosity predicted from variant allele frequency.

Colored bars represent the mean zygosity frequency measured in single cells (847 single-cell measurements total). White bars with black outline represent the predicted mean zygosity frequency we expect our method to report for a sample for which all single cells are heterozygous. Actual genotype data is for N = 12 arrays of OCI-AML3 cells. Predicted genotype data is from measurements of single plasmids with one copy of each wild-type and mutant allele. Predicted genotype data are for N = 6 arrays of these heterozygous plasmids. In each case, genotype frequencies were quantified in each array; reported means were calculated between arrays. Error bars represent standard deviations of these measurements.