Matrilineal inheritance of wild-type alleles in progeny of females carrying yReMEDE.

Mating scheme for generation of F1 yReMEDE females and their F2 progeny. Images of fly heads indicate scored phenotypes of eye fluorescence (white = no marker, green = EGFP, red = RFP) and body color (brown = + or +Δ, yellow = Drive or -Δ). Possible allelic combinations are shown below head images (ReMEDE = Drive, wild type = +, in-frame indel = +Δ, out-of-frame indel = -Δ). Male flies are indicated by the presence of the Y chromosome. Blue boxes enclose genotypes and phenotypes that confirm SSA-mediated transgene excision, regardless of the PAM sequence.

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