Mating type genotype and assay of 22 ∆ 10α.

(a) Genotyping PCR of 22 ∆ 10α and 23344c strains with oligonucleotide pairs that correspond to the Mata (a) or Matα (α) mating type. Expected sizes are Mata: 544 bp and Matα: 404 bp. (b) Mating assay between 23344c, 22 ∆ 8AA and 22 ∆ 10α with Y187 and AH109. Mated cells were streaked on SD medium supplemented with Met, Ade, Trp, Leu, and with or without Ura and His. Diploid cells, resulting from mating of cells of different mating types should be both Ura and His prototroph. Two colonies of each of the tested strains were mated with AH109 and Y187.