Male mantle cell viability and proliferation in different culture media.

A) Examples of images obtained for counting cells from one culture at the indicated time point. Nuclei were stained with Hoescht dye and cells were counted using automatic detection. Five frames from triplicate cultures were counted for every sample. For space concerns, only a fraction of the complete image is shown. Scale bar = 25μm. B, C) Relative number of cells per frame compared to the number of cells that remained attached after the first change of media (Day 1) in each medium at 15 (B) and 25°C (C) after the indicated number of days in culture. Within each box, bold horizontal lines denote median values; boxes extend from the 25th to the 75th percentile of each group’s distribution of values; vertical extending lines denote the 5th and 95th percentiles.