Macrophage phenotypes change over time during type II and type III infection.

At 5, 10, and 21 dpi, immune cells were isolated from the spleen of either type II- or type III-infected mice and stained and analyzed as in Fig 3. A,B. Quantification of the frequency and number of M2 (A) or M1-like (B) macrophages at 5 dpi. C,D. Quantification of the frequency (C) and number (D) of splenic M2 macrophages over time. E,F. Quantification of the frequency (E) and number (F) of splenic M1-like macrophages over time. G,H. Quantification of the frequency (G) and number (H) of splenic Tregs over time. Bars, mean ± SEM. N = 5–7 mice/infected group. *p<0.05, **p<0.01, two-way ANOVA with Fisher’s protected LSD. Data representative of 2 independent experiments. ● = type II, ■ = type III.