Loss of AeAct-4, myo-fem, or Aeflightin results in flightlessness.

(A) Blinded workflow used to score flight ability without experimenter knowledge of genotype, with subsequent genotyping assays. Photographs of wild type (B), AeAct-4 ^Δ10/Δ10 (C), myo-fem ^Δ11/Δ11 (D), or Aeflightin^Δ4/Δ5 (E) females when resting. The percentage of flightless male (black bars) or female (grey bars) mosquitoes for AeAct-4 (F), myo-fem (G), and Aeflightin (H) based on each genotype. The number above each bar represents the number of individuals displaying the flightless phenotype and were confirmed for the specified genotype via sequencing. The dotted red line indicates 100% flightless.