Knockdown of FBLN1 can promote ferroptosis in LPS-induced ALI.

(A) qRT-PCR was used to detect the knockdown efficiency of FBLN1 in MLE-12 cells. (B) WB was used to detect the knockdown efficiency of FBLN1 in MLE-12 cells. (C) An ROS kit was used to detect the lipid ROS level of MLE-12 cells after 1 μg/mL LPS+si-FBLN1 treatment. (D) An MDA kit was used to detect the changes in the MDA concentration of MLE-12 cells after 1 μg/mL LPS+si-FBLN1 treatment. (E) A GSH kit was used to detect the changes in the GSH concentration of MLE-12 cells after 1 μg/mL LPS+si-FBLN1 treatment. (F) Fe²⁺ kit was used to detect the changes in the Fe²⁺ concentration of MLE-12 cells after 1 μg/mL LPS+si-FBLN1 treatment. ALI, Acute lung injury; CCK-8, Cell counting kit-8; LPS, lipopolysaccharide; qRT-PCR, Quantitative real-time polymerase chain reaction; WB, Western blot; MDA, malondialdehyde; GSH, glutathione; ROS, reactive oxygen species. *p<0.05.

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