Key neuronal transcripts show an increase in steady-state levels in Rrp40 mutant flies.

(A) An Integrative Genomic View (IGV) screenshot (http://software.broadinstitute.org/software/igv/) of Arc1 track peaks across the Drosophila genome (dm6 assembly) in Rrp40 mutants [Rrp40 D87A/D87A (Upper) and Rrp40 G11A/G11A (Middle)] as well as Rrp40-WT (WT, Lower). Chromosome number and location (red rectangle; top) are displayed at the top. (B,C) Changes in the level of representative transcripts were validated using RT-qPCR in Rrp40-WT and Rrp40 mutant flies. Data are presented as the Fold Change Normalized to Act5c transcript (which was unchanged in the RNA-Seq datasets) where the value for the Rrp40-WT sample is set to 1.0. Results are presented as mean ± standard error of the mean (*p<0.05;**p<0.01;***p<0.001;****p<0.0001 vs. the WT group in a two-tailed t-test). (D) Expression of human EXOSC3 (UAS-EXOSC3-myc) in neurons rescues the increased steady-state levels of the Arc1 (left) and WFS1 (right) transcripts in both Rrp40 mutants (G11A/G11A or D87A/D87A). The steady-state levels of the Arc1 and WFS1 transcripts were analyzed using RT-qPCR in control Rrp40-WT flies (set to 1.0) and mutant flies expressing human EXOSC3 (+Gal4) or not (-). Data are presented as the means ± standard error of the mean (*p<0.05;**p<0.01 vs. the WT group in a two-tailed t-test).