pone.0203521.g005.tif (843.96 kB)

Influenza HA specifically attenuates innate cytokines in HKSP-treated cells.

figure

posted on 2018-09-07, 17:53 authored by Sinead T. Loughran, Patrick A. Power, Paula T. Maguire, Samantha L. McQuaid, Paul J. Buchanan, Ingileif Jonsdottir, Robert W. Newman, Ruth Harvey, Patricia A. Johnson

The levels of (A) IL-6, IL-23, (B) IL-12p70, (C) IL-27, (D) IL-1β, (E) IL-10 and (F) TGF-β secreted by CD14⁺ cells following 24 h treatment with HKSP, H1N1 HA (1μg ml^-1 or 3 μg ml^-1) or H3N2 HA (1μg ml^-1 or 3 μg ml^-1) alone or in combination with HKSP (or untreated as a control) were established by ELISA. Each column represents normalised mean cytokine levels + SEM of at least 3 experimental repeats of each treatment/donor. Each ELISA represents normalised results from multiple donors: (A) IL-6 (n = 3), IL-23 (n = 4), (B) IL-12p70 (n = 3), (C) IL-27 (n = 6), (D) IL-1β (n = 4), (E) IL-10 (n = 4), and, TGF-β (n = 3). Statistical analyses were performed to compare cytokine levels in cells exposed to HKSP versus cells exposed to H1N1 HA or H3N2 HA (1μg ml^-1 or 3 μg ml^-1) in combination with heat-inactivated HKSP, by fitting a One-way ANOVA to the data and using a Sidak test to adjust for multiple testing (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).