pone.0232230.g005.tif (1.84 MB)

Infiltration of neutrophils and tumor angiogenesis in orthotopic tumors.

figure

posted on 2020-04-27, 17:30 authored by Keizo Takenaga, Miho Akimoto, Nobuko Koshikawa, Hiroki Nagase

(A) The amount of CXCL3 in Panc02-shCont (n = 9) and Panc02-sh#5 (n = 9) tumor tissues. (B) IHC analysis of MPO⁺ neutrophil infiltration in Panc02-shCont and Panc02-sh#5 tumors. Bar: 50 μm. (C) The number of MPO⁺ cells per field (×400 magnification) in Panc02-shCont (n = 9 fields of 2 tissue sections) and Panc02-sh#5 tumors (n = 12 fields of 2 tissue sections). (D) RT-qPCR analysis of expression of the neutrophil marker gene Ly6G in Panc02-shCont (n = 5 tumors) and Panc02-sh#5 tumors (n = 6 tumors). (E) The number of F4/80⁺ cells per field (×400 magnification) in Panc02-shCont (n = 10 fields of 5 tissue sections) and Panc02-sh#5 tumors (n = 12 fields of 4 tissue sections). (F) The number of CD20⁺ cells per field (×400 magnification) in Panc02-shCont (n = 9 fields of 3 tissue sections) and Panc02-sh#5 tumors (n = 10 fields of 3 tissue sections). (G, H) Angiogenesis in Panc02-shCont and Panc02-sh#5 tumors. G: CD31 staining. Bar: 50 μm. H: Vessel density. Panc02-shCont (n = 11 fields) and Panc02-sh#5 cells (n = 14 fields). The data are shown as the mean ± SD. *P<0.05. ns, not significant.