ppat.1008473.s002.tif (16.44 MB)

Infection by parental and RHIM mutant VZV.

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posted on 10.07.2020 by Megan Steain, Max O. D. G. Baker, Chi L. L. Pham, Nirukshan Shanmugam, Yann Gambin, Emma Sierecki, Brian P. McSharry, Selmir Avdic, Barry Slobedman, Margaret Sunde, Allison Abendroth

(A) Average percentage of VZV-infected cells in the parental and VZV RHIM mutant virus HT-29 cultures at the beginning of each viability assay as determined by flow cytometry staining for the gE:gI glycoprotein complex. Error bars show standard error of the mean, from 4 independent replicates. (B) Viability of mock, VZV and VZV RHIM deleted (VZV-RHIMKO) virus infected HT-29s (72 h post-infection) following treatments with TNF (T; 30 ng/ml), BV-6 (S; 1 μM), z-VAD-fmk (V; 25 μM) and necrostatin-1 (Nec1; 30 μM) alone or in combination as indicated. Data was normalised to DMSO only control. Error bars show standard error of the mean, from 3 independent replicates and statistical significance was determined using a two-way ANOVA. C. Average percentage of VZV-infected cells in the parental and VZV RHIM deleted virus HT-29 cultures at the beginning of each viability assay as determined by flow cytometry staining for the gE:gI glycoprotein complex. Error bars show standard error of the mean, from 3 independent replicates.

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