Infection by parental and RHIM mutant VZV.

(A) Average percentage of VZV-infected cells in the parental and VZV RHIM mutant virus HT-29 cultures at the beginning of each viability assay as determined by flow cytometry staining for the gE:gI glycoprotein complex. Error bars show standard error of the mean, from 4 independent replicates. (B) Viability of mock, VZV and VZV RHIM deleted (VZV-RHIMKO) virus infected HT-29s (72 h post-infection) following treatments with TNF (T; 30 ng/ml), BV-6 (S; 1 μM), z-VAD-fmk (V; 25 μM) and necrostatin-1 (Nec1; 30 μM) alone or in combination as indicated. Data was normalised to DMSO only control. Error bars show standard error of the mean, from 3 independent replicates and statistical significance was determined using a two-way ANOVA. C. Average percentage of VZV-infected cells in the parental and VZV RHIM deleted virus HT-29 cultures at the beginning of each viability assay as determined by flow cytometry staining for the gE:gI glycoprotein complex. Error bars show standard error of the mean, from 3 independent replicates.

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