In vitro kinase assay identifies AHR as the PLK1 substrate.
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posted on 2023-11-21, 18:40 authored by Chaohao Li, Derek B. Allison, Daheng He, Fengyi Mao, Xinyi Wang, Piotr Rychahou, Ibrahim A. Imam, Yifan Kong, Qiongsi Zhang, Yanquan Zhang, Jinghui Liu, Ruixin Wang, Xiongjian Rao, Sai Wu, B. Mark Evers, Qing Shao, Chi Wang, Zhiguo Li, Xiaoqi LiuA, Fragmentation of full-length AHR ORF into F1, F2, F3, and F4 based on its functional domains. The C-termini of each fragment is tagged with glutathione s-transferase (GST). NLS, nuclear localization signal. NES, nuclear export signal. PAS, Per-Arnt-Sim domain. TAD, transactivation domain. Created with BioRender.com. B, In vitro kinase assay with four fragments. Coom, Coomassie Blue. 32P, radioactive phosphorus-32. The final concentration of ONV is 50nM. C, Previous identified consensus phosphorylation motif of PLK1 substrates and S489 site of F3. D, Potential PLK1 phosphorylation sites identified using the software. E, In vitro kinase assay with WT or mutated F3. F, In vitro kinase assay with WT and SA mutated F3 or HA-AHR.
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