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Immunostaining for p-Mad in Mcm5 mutants.

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posted on 23.06.2022, 20:05 authored by Irene Rubio-Ferrera, Pablo Baladrón-de-Juan, Luis Clarembaux-Badell, Marta Truchado-Garcia, Sheila Jordán-Álvarez, Stefan Thor, Jonathan Benito-Sipos, Ignacio Monedero Cobeta

Immunostaining for p-Mad in Control (A) and Mcm5 mutants (B) at stage 18h AEL. (C) Quantification of Cas positive Ap cluster cells in control and mcm5 mutant (U-Mann-Whitney test; n≥3 CNS per genotype, n≥ 18 hemisegments per genotype, n.s = non-significant). Immunostaining for proFMRFa, Eya and pMad in Control (D) and expression of wild type forms of the type I BMP receptors (sax and tkv) in Mcm5 mutants (E), at stage 18h AEL. Genotypes: (A,D) w1118 (B) Mcm5exc222/Mcm5exc222, (E) pros-Gal4/UAS-sax, UAS-tkv; Mcm5exc222.

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