Immunohistochemical quantification of cell infiltration into allografts, syngrafts and normal skin (NS).
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posted on 2017-07-26, 17:36 authored by Or Friedman, Narin Carmel, Meirav Sela, Ameen Abu Jabal, Amir Inbal, Moshe Ben Hamou, Yakov Krelin, Eyal Gur, Nir ShaniSamples (n = 3–5 rats per group) from the graft-recipient interface of a syngraft, allograft or from NS were removed at the indicated times and paraffin sections were stained with anti CD68, CD8 or CD4 antibodies followed by hematoxylin counterstaining. Stained cells were counted in 10 fields and the average stained cells per field is displayed in the graphs. * P<0.05 and ** P<0.01.
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3CLpost-VCA responsesallogeneic vascularized groin flap transplantationsallogeneic vascularized groin flapanti-alloantigen adaptiveday 5 post-transplantationorgan rejectionstage rejectionface vascularizedCCLPCRwound healing-associated genesResults Immune cell infiltrationprotein analysesrejection phasewound sitePost-operative days 2allograft rejection processesVCA rejection ratesIL -10VCA rejectionCXCL5 dayscell infiltrationdonor-recipient interfaceallograft rejectionCXdamage siteConclusions High resemblancetransplant acceptancerat model Background Handcell infiltration spreadbroad-scope gene
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