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Immunofluorescence staining of <i>Slc17a7</i> single KO and <i>Slc17a7</i>, <i>Slc17a6</i>, and <i>Slc17a8</i> triple KO β-cell lines.

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posted on 2017-11-01, 17:42 authored by Naoya Murao, Norihide Yokoi, Kohei Honda, Guirong Han, Tomohide Hayami, Ghupurjan Gheni, Harumi Takahashi, Kohtaro Minami, Susumu Seino
<p>Green, Insulin; red, VGLUT1 (A) or VGLUT2 (B); blue, 4',6-diamidino-2-phenylindole (DAPI). Scale bars, 50 μm.</p>

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    Slc 17aGIPincretin-induced insulin secretionaspartate aminotransferase 1β- cell glutamatecytosolic glutamate productionpotentiate insulin secretionIIISpancreas perfusion experimentβ- cell linesMApancreatic β- cellsSlc 17a genesGLPASTglutamate transportersVGLUTSlc 17a KO miceinsulin granule exocytosisincretin-induced insulin secretion Incretinscytosolic glutamateSingle Slc 17a KO β- cell linesCRISPRwild-type Slc 17a

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