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HPLC profile of rhEPO in transformed and control hairy root cultures.

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posted on 2017-08-11, 17:29 authored by Poornima Devi Gurusamy, Holger Schäfer, Siva Ramamoorthy, Michael Wink

(A) Positive control rhEPOCHO standard recombinant human erythropoietin from CHO cells. (B) Hairy root culture pK7cal/rhEPOHR. (C) Negative control hairy root culture Nt/ATCCHR. The arrow indicates the presence of peaks.

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    ER signal peptideARPEFWHPLCCaco -2 cellspigment epithelial cellsNicotiana tabacum LHairy root cultureC-terminal hexahistidine tag185.48 pg rhEPO HR gT 0 generation plantlets regeneratedDNAamount 14.8 ng groot culturesRNAvector pK 7 WG 2Dprotein66.75 ng groot culture systemcal signal peptide

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