Generation of galET-short 3’ ends by RNase III-mediated transcript cleavage.

A) 3’RACE assay of galET 3’ends in E. coli mutants that are deficient in exoribonucleases. Strains used are MG1655Δrnb (where the gene for RNase II is deleted from the chromosome) (lane 2), MG1655Δrnr (where the gene for RNase R is deleted from the chromosome) (lane 3), W3110Δpnp (where the gene for PNPase is deleted from the chromosome) (lane 5). The results of the WT for strains Δrnb and Δrnr are shown in lane 1, and for Δpnp strain is shown in lane 4. B) 3’RACE assay of the galET mRNA from the cell-free system in presence of pRNIII plasmid, where the gene for RNase III is under control of the T7 promoter. The data presented in these blots are representative of three independent experiments.