posted on 2021-12-08, 18:29authored byNadine Artelt, Alina M. Ritter, Linda Leitermann, Felix Kliewe, Rabea Schlüter, Stefan Simm, Jens van den Brandt, Karlhans Endlich, Nicole Endlich
(A) The specific palladin KO was confirmed by immunohistochemistry staining of paraffin kidney sections. PodoPalld129+/+ mice exhibit strong palladin-expressing podocytes (arrow). In contrast, there is no palladin signal (arrowhead) in PodoPalld129-/- podocytes. Scale bar represents 10 μm. (B) In addition, the palladin KO was verified by qRT-PCR (mean±SD, ***p<0.001; 6 months: Mann-Whitney U test, 12 months: unpaired Student’s t-test).