Fungal genera containing relevant phytopathogens combated with effective fungicides.

Fusarium/Gibberella spp. were not effectively suppressed by fungicide application and accumulated in all intensive treatments, particularly in pre-crop maize samples (WW1). In contrast, Mycospharella/Septoria spp. were only enriched in pre-crop rapeseed samples (WW2) and more abundant in the MP extensive treatment that never received fungicides in the long-term field trial. Fusarium/Gibberella were detectable with the ITS1 (designated with a red dot) and ITS2 (designated with a blue triangle) primers, and Mycosphaerella/Septoria only with the ITS1 primers. For abbreviations refer to Fig 3.