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posted on 13.05.2022, 17:43 by Parinda Thayanukul, Benchaporn Lertanantawong, Worachart Sirawaraporn, Surat Charasmongkolcharoen, Thanyarat Chaibun, Rattanalak Jittungdee, Pattamaporn Kittayapong

Detection of Wolbachia wAlbB gene in different mosquito species using LAMP assay with HNB indicator (A) and ethidium bromide-stained gel (B): (1) no template control, (2) wild-type Aedes aegypti (AegW, Aae-JJ), (3) Wobachia trans-infected Thai Ae. aegypti (AegB, wAlbB-TH), (4) Aedes albopictus (Alb, Aal-CH), and (5) Culex quinquefasciatus (Cq-BK). M is 1 kb plus DNA Ladder from Invitrogen. The condition for LAMP reaction was 6.4 units of Bst 2.0 DNA polymerase, 65°C for 60 min and 80°C for 10 min.