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posted on 13.05.2022, 17:43 by Parinda Thayanukul, Benchaporn Lertanantawong, Worachart Sirawaraporn, Surat Charasmongkolcharoen, Thanyarat Chaibun, Rattanalak Jittungdee, Pattamaporn Kittayapong

Detection of Wolbachia wAlbB gene in different mosquito species using LAMP assay with HNB indicator (A) and ethidium bromide-stained gel (B): (1) no template control, (2) wild-type Aedes aegypti (AegW, Aae-JJ), (3) Wobachia trans-infected Thai Ae. aegypti (AegB, wAlbB-TH), (4) Aedes albopictus (Alb, Aal-CH), and (5) Culex quinquefasciatus (Cq-BK). M is 1 kb plus DNA Ladder from Invitrogen. The condition for LAMP reaction was 6.4 units of Bst 2.0 DNA polymerase, 65°C for 60 min and 80°C for 10 min.

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Simple, sensitive, and cost-effective detection of wAlbB Wolbachia in Aedes mosquitoes, using loop mediated isothermal amplification combined with the electrochemical biosensing method
PLOS Neglected Tropical DiseasesPLOS Neglected Tropical Diseases

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showed high sensitivityhuman hosts highlightsdna strand displacementcrude dna extractsbecame less capableseveral arboviral diseasesdengueborne viral diseasesbased electrochemical sensordetection limit reducedelectrochemical biosensing methodaedes aegypti </lamp assay combinedarboviral diseasesaegypti </aedes </based approachesroutine detectioneffective detectiondetection capacitywolbachia </w </ae </yellow feverwolbachia -</vectorial competencetransmitting arbovirusesstudy demonstratesresults suggestlamp techniquefield surveillancedeployed widely4 nm

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