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Fibrillation kinetics of insulin incubated in the presence of degradation products of aloin.

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posted on 2016-02-16, 07:56 authored by Eva S. Lobbens, Vito Foderà, Nils T. Nyberg, Kirsten Andersen, Anna K. Jäger, Lene Jorgensen, Marco van de Weert

Representative, normalized fibrillation curves of insulin in the presence of (A) 2% DMSO (green), 400 μM aloin in DMSO (black) and 400 μM aloin heated 10 minutes in DMSO (blue). (B) 0.4% EtOH (red), aloin incubated 1 week in PBS buffer (blue), (C) 2% DMSO (green), 400 μM aloe-emodine (blue) or 400 μM D-glucose (black), (D) 2% DMSO (green), 2% CH2Cl2 (purple), the CH2Cl2 phase of a 3 weeks old 400 μM aloin solution incubated with CH2Cl2 (blue) and the dried CH2Cl2 phase reconstituted in DMSO (black). Fibrillation conditions: ThT assay, pH 7.4.

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