Expression of IL-33, ST2L, cytokines and chemokines in orthotopic tumors.

(A) RT-qPCR analysis of Il33 and ST2L in Panc02-shCont and Panc02-sh#5 tumors (n = 3). (B) Gene expression profiling of cytokines and chemokines between Panc02-shCont and Panc02-sh#5 tumors were performed using a PCR array. (C) RT-qPCR analysis of various chemokine (C-X-C motif) ligands and Cxcr2 in Panc02-shCont and Panc02-sh#5 cells (n = 3) and tumors (n = 7–9). (D) RT-qPCR analysis of Cxcl13 in Panc02-shCont (n = 7) and Panc02-sh#5 tumors (n = 9). The data are shown as the mean ± SD. *P<0.05. **P<0.001. ns, not significant.