Example sequences from the case study.

Showing DNA and protein sequence of BfpE, the linker L0 and His tag at N-terminus and C-terminus are depicted (A). (B, C, D), showing sequence of interest of BfpD, BfpU and BfpD respectively and the target deletion is selected by arrows. Cloning sites NdeI and BamHI (underlined) of pET15TEV and BfpD (N-Term) are shown in Fig (E).